Peptide Reconstitution Guide

Peptide Reconstitution Protocol

Reconstitution is the process of dissolving lyophilized (freeze-dried) peptide powder into solution for research use. Proper technique preserves peptide integrity and ensures accurate dosing throughout the vial’s usable life.

Step 1: Gather Supplies

You will need: lyophilized peptide vial, bacteriostatic water (or sterile water for GHK-Cu), alcohol swabs, insulin syringe (U-100), and a clean workspace.

Step 2: Calculate Volume

Use our reconstitution calculator to determine the exact volume of solvent needed for your target concentration.

Step 3: Add Solvent Slowly

Inject the solvent against the glass wall of the vial — never directly onto the lyophilized cake. Allow the solution to dissolve naturally. Do NOT shake or vortex. Gentle swirling is acceptable if needed after 2-3 minutes.

Step 4: Storage

Store reconstituted peptides at 2-8°C (refrigerator). Most peptides remain stable for 14-28 days after reconstitution. See individual peptide profiles for specific stability data.

Important Notes

GHK-Cu exception: Use sterile water (NOT bacteriostatic water) for GHK-Cu reconstitution. The benzyl alcohol preservative in BAC water chelates the copper ion, reducing biological activity.